RESEARCH TECHNICIAN II

RESEARCH TECHNICIAN II

Description:
Job Description

Provide operational supports to the Director/Manager of Islet Isolation cGMP Laboratory at MGH. Be trained on and adhere to Standard Operating Procedures (SOPs) that apply to human islet manufacture preparation; manufacturing; conducting QC assays and laboratory operation.

Provide technical support to activities that pertain to the human islet manufacturing processes and quality control assays. Perform large animal and rodent islet isolation and quality control assays. Perform duties related to basic science research.

JOB SPECIFIC RESPONSIBILITIES:

Human Islet Manufacturing Supporting Responsibilities

In conjunction with other team members or independently

Receive, inspect, document, collect COA or certificate of compliance/sterilization, and store supplies as indicated in SOPs. Washing and sterilization of reusable items such as screens, gradient makers, marbles, digestion chamber and etc as indicated in SOPs. Restock the supplies after each islet isolation

Organize the facilitys furniture, equipment, cabinet and shelves in a professional way. Document all equipment maintenance log; Maintain all the incubators and waters bath as indicated in SOPs. Remove waste and organize islet isolation facility after each isolation as indicated in SOPs.

Supervise the contractor who provides the monthly facility clean, documentation of the service work as indicated in SOPs: Perform the weekly clean room disinfect procedure as indicated in SOPs. Exchange surgical gowns for the team

Check facility monitoring system on daily basis and document any unusually activities based on SOP.

Human Islet Manufacturing Responsibilities

In conjunction with other team members or independently

Working closely with team member in all activities that pertain to the human islet manufacturing processes required for clinical islet transplantation.

Setting up the laboratory for islet manufacture as indicated in SOPs; Performing environmental monitoring( viable and no-viable); Cleaning hoods before, during and after the isolations as indicated in the SOP

Preparing the solutions for islet isolation and handling the tissue as indicated in SOPs.

Deliver microbiology samples to the Microbiology Lab of MGH.

Preparing the FDA-PI, Dithionate dye before each isolation.

Culturing and Distributing tissue to investigator as indicated in SOPs.



Quality Control Responsibilities.

Treat INS, DNA and histology samples as indicated in SOPs. Labeling and storage samples as indicated in SOPs.

Perform Glucose Stimulated Insulin Release as indicated in SOPs

Perform INS quantification assay(ELISA)as indicated in SOPs

Perform DNA quantification assay as indicated in SOPs

Perform Endotoxin assay as indicated in SOPs

Submit original test results to the Director/Manager of the islet isolation laboratory.

Large Animal and Rodent Islet Isolation.

Work closely with team member or independently to perform all activities that pertain to the large animal pancreases procurement and islet isolation.

Prepare solutions needed for islet isolation; Organize the lab before and after each isolation

Culture and Distribute tissue to investigator. Perform bioassays or tests for quality control.

Data management Responsibilities:

Enter isolation data into labs database.

Enter islet distribution data into labs data basis

Basic Science research:

Execute protocols of routine molecular and immunology assays

Perform basic design and modification of protocols when necessary

Organize and summarize acquired data using scientific and statistical techniques

Prepare and present reports of experiments

MINIMUM REQUIREMENTS:

EDUCATION: BS degree or four years of college or equivalent education and experience is required
Qualifications:

Company Profile:
Founded in 1811, Massachusetts General Hospital is the third-oldest general hospital in the country, and the oldest and largest in New England. The 907-bed world-renowned medical center offers sophisticated diagnostic and therapeutic care in virtually every specialty and subspecialty of medicine and surgery. The largest teaching hospital of Harvard Medical School, MGH is on the cutting edge of patient care, research and medical education. It is the largest nongovernment employer in the city of Boston, with more than 21,500 employees.

MGH is among the leading medical institutions continuously recognized by U.S. News & World Report's annual honor roll of America's best hospitals. In 2009, MGH ranked fifth nationally, with MGH Psychiatry ranking first in that specialty for the 14th consecutive year. MGH has been named to the list since the publication began conducting the survey in 1990.

In 2008, MGH again was named a Magnet hospital by the American Nurses Credentialing Center, a subsidiary of the American Nurses Association. The Magnet Recognition Program recognizes health care organizations that provide the best in nursing care and to uphold the tradition of excellence in professional nursing practice. MGH was the first Massachusetts hospital to earn the Magnet designation in 2003.

MGH has repeatedly been named an employer of choice. Most recently, it was named to The Globe 100, a list of the top employers in Massachusetts compiled by The Boston Globe. The hospital has also earned a spot on Conceive magazine's 2009 list of the Top 50 Workplaces for Prospective Parents.

A career with MGH means you'll be part of a rich legacy of providing top-quality patient care and an integral part of ensuring that our success continues. Located in Boston, MGH is accessible by several MBTA lines. Our benefits and perks are outstanding and include health/dental insurance, a retirement plan, 403b, paid time off, tuition reimbursement, childcare center and a fitness center. There is only one MGH! For more information, please visit us at .

Massachusetts General Hospital is an Equal Employment/Affirmative Action Employer.